Huntingtin (Htt) localizes to endosomes, but its role in the endocytic pathway is not established.
Recently, we found that Htt is important for the activation of Rab11, a GTPase involved in …

The biological function of receptors is determined by their appropriate trafficking through the
endosomal pathway. Following internalization, the transferrin (Tf) receptor quantitatively …

… Bhogaraju and colleagues determined experimentally that the human IFT81 N-terminus and
the IFT81N–IFT74N heterodimer bound tubulin with a K d of 16 µM and 0.9 µM, respectively…

Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures
containing enhanced green fluorescent protein-tagged glucose transporter 4 (GLUT4) within a …

… Cell lysates were mixed thoroughly and 20–80 μl of lysate was added to a bDNA capture
plate along with 0–60 additional diluted lysis mixture without proteinase K to fill up to 80 μl total …

The protein nephrocystin-4 (NPHP4) is widespread in ciliated organisms, and defects in
NPHP4 cause nephronophthisis and blindness in humans. To learn more about the function of …

… Custom software was used to calculate the three-dimensional image of a model smooth
muscle cell filled with 50 μM fluo-3 (K d = 390 nM) in equilibrium with a resting [Ca 2+ ] of 100 nM…

… Clusters were generated in the 103 Cell Ranger software via K-means clustering based on
the principal-component analysis (PCA) of the transcriptomic signature. Cluster names were …

… path is the same), there is a scale factor λ K relating the intensity of the TIRF image λ I TIRF
to … The value of z 0 depends on d and K and is constant as long as the microscope set up is …

Beclin 1 is a haploinsufficient tumor suppressor that is decreased in many human tumors.
The function of beclin 1 in cancer has been attributed primarily to its role in the degradative …