Abstract
Functional maturation of GABAergic innervation in the developing visual cortex is regulated by neural activity and sensory inputs and in turn influences the critical period of ocular dominance plasticity. Here we show that polysialic acid (PSA), presented by the neural cell adhesion molecule, has a role in the maturation of GABAergic innervation and ocular dominance plasticity. Concentrations of PSA significantly decline shortly after eye opening in the adolescent mouse visual cortex; this decline is hindered by visual deprivation. The developmental and activity-dependent regulation of PSA expression is inversely correlated with the maturation of GABAergic innervation. Premature removal of PSA in visual cortex results in precocious maturation of perisomatic innervation by basket interneurons, enhanced inhibitory synaptic transmission, and earlier onset of ocular dominance plasticity. The developmental and activity-dependent decline of PSA expression therefore regulates the timing of the maturation of GABAergic inhibition and the onset of ocular dominance plasticity.
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Acknowledgements
We thank P. Wu, E. Putignano, N. Berardi and J. Boehm for technical assistance and discussion; and E. Ruthazer for critically reading the manuscript. This work was supported by the National Institutes of Health (RO1 EY 13564-01). G.D.C. has a National Association for Research in Schizophrenia and Depression (NARSAD) Young Investigator Award founded by the Forrest C. Lattner Foundation. Z.J.H. is a Pew and McKnight Scholar.
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G.D.C. and Z.J.H. conceived and organized the project and wrote the manuscript. G.D.C. conducted most of the experiments. B.C. contributed to the in vitro and in vivo morphological studies. S.J.K. and Y.F. carried out the mPSC recordings. M.-C.B. performed the western blotting. C.Z.W. contributed to the PSA expression analysis in vivo. U.R. provided endoN and advice. L.M. provided the facility for dark rearing and VEP recording.
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Di Cristo, G., Chattopadhyaya, B., Kuhlman, S. et al. Activity-dependent PSA expression regulates inhibitory maturation and onset of critical period plasticity. Nat Neurosci 10, 1569–1577 (2007). https://doi.org/10.1038/nn2008
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DOI: https://doi.org/10.1038/nn2008