Abstract
Although the requirement of neurotrophins for the prevention of cell death in the peripheral nervous system is well established, their physiological involvement in nerve growth is still unclear. To address this question, we generated a mouse that expresses the green fluorescent protein in post-mitotic neurons, allowing the repeated visualization of all motor and sensory axons during development. We imaged the growth of these axons into the limb bud of day 10.5 embryos. Sensory axons, but rarely motor axons, were targeted to ectopically placed beads containing any of the neurotrophins NGF, BDNF, NT-3 or NT-4/5. Conversely, a combination of function-blocking monoclonal antibodies to NGF, BDNF and NT-3 dramatically inhibited elongation of both sensory and motor axons in the limb bud, indicating that the growth of mixed nerves is dependent upon neurotrophins during development.
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Acknowledgements
We thank R. Jaenisch for the J1 cells, N. Hirokawa for the Mtapt genomic clones, B. Kunkel for blastocyst injections, H. Breitsameter and M. Rittirsch for animal care, G. Dechant, M. Korte, and F. Maina for comments, and L. Lindemann for the anti-myc antibody. K.L.T. was supported by a post-doctoral fellowship from the European Molecular Biology Organization.
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Tucker, K., Meyer, M. & Barde, YA. Neurotrophins are required for nerve growth during development. Nat Neurosci 4, 29–37 (2001). https://doi.org/10.1038/82868
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DOI: https://doi.org/10.1038/82868
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