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Krox-20 controls myelination in the peripheral nervous system

Abstract

THE molecular mechanisms controlling the process of myelination by Schwann cells remain elusive, despite recent progress in the identification and characterization of genes encoding myelin components (reviewed in ref. 1). We have created a null allele in the mouse Krox-20 gene, which encodes a zinc-finger transcription factor2,3, by in-frame insertion of the Escherichia coli lacZ gene, and have shown that hindbrain segmentation is affected in Krox-20−/− embryos4. We demonstrate here that Krox-20 is also activated in Schwann cells before the onset of myelination and that its disruption blocks Schwann cells at an early stage in their differentiation, thus preventing myelination in the peripheral nervous system. In Krox-20−/− mice, Schwann cells wrap their cytoplasmic processes only one and a half turns around the axon, and although they express the early myelin marker, myelin-associated glycoprotein5, late myelin gene products are absent, including those for protein zero6 and myelin basic protein7. Therefore Krox-20 is likely to control a set of genes required for completion of myelination in the peripheral nervous system.

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References

  1. Lemke, G. Glia 7, 263–271 (1993).

    Article  CAS  Google Scholar 

  2. Chavrier, P. et al. EMBO J. 7, 29–35 (1988).

    Article  CAS  Google Scholar 

  3. Chavrier, P. et al. EMBO J. 9, 1209–1218 (1990).

    Article  CAS  Google Scholar 

  4. Schneider-Maunoury, S. et al. Cell 75, 1199–1214 (1993).

    Article  CAS  Google Scholar 

  5. Trapp, B. D. & Quarles, R. H. J. Cell biol. 92, 877–882 (1982).

    Article  CAS  Google Scholar 

  6. Greenfield, S., Brostoff, S., Eylar, E. H. & Morell, P. J Neurochem. 20, 1207–1216 (1973).

    Article  CAS  Google Scholar 

  7. Kies, M. W., Murphy, J. B. & Alvord, E. C. in Chemical Pathology of the Nervous System (ed. Folch-Pi, J.) 197 (Pergamon, London, 1961).

    Google Scholar 

  8. Wilkinson, D., Bhatt, S., Chavrier, P., Bravo, R. & Charnay, P. Nature 337, 461–464 (1989).

    Article  ADS  CAS  Google Scholar 

  9. Jessen, K. R. et al. Neuron 12, 509–527 (1994).

    Article  CAS  Google Scholar 

  10. Gabe, M. Techniques histologiques (ed. Masson) 468 (Masson, Paris, 1968).

    Google Scholar 

  11. Uyemura, K., Tobari, C. & Hirano, S. Biochim. biophys. Acta 214, 190–197 (1970).

    Article  CAS  Google Scholar 

  12. Brockes, J. P., Fields, K. L. & Raff, M. C. Brain Res. 165, 105–118 (1979).

    Article  CAS  Google Scholar 

  13. Bunge, M. B., Bunge, R. P., Kleitman, N. & Dean, A. C. Devl Neurosci. 11, 348–360 (1989).

    Article  CAS  Google Scholar 

  14. Trapp, B. D., Quarles, R. H. & Suzuki, K. J. Cell biol. 99, 594–605 (1984).

    Article  CAS  Google Scholar 

  15. Trapp, B. D. J. Cell Biol. 107, 675–685 (1988).

    Article  CAS  Google Scholar 

  16. Li, C. et al. Nature 369, 747–750 (1994).

    Article  ADS  CAS  Google Scholar 

  17. Giese, K. P., Martini, R., Lemke, G., Soriano, P. & Schachner, M. Cell 71, 565–576 (1992).

    Article  CAS  Google Scholar 

  18. Filbin, M. T., Walsh, F. S., Trapp, B. D., Pizzey, J. A. & Tennekoon, G. I. Nature 344, 871–872 (1990).

    Article  ADS  CAS  Google Scholar 

  19. Kirschner, D. A. & Ganser, A. L. Nature 283, 207–210 (1980).

    Article  ADS  CAS  Google Scholar 

  20. Kimura, M. et al. Proc. natn. Acad. Sci. U.S.A. 86, 5661–5665 (1989).

    Article  ADS  CAS  Google Scholar 

  21. Owens, G. C. & Bunge, R. P. Glia 3, 118–124 (1990).

    Article  CAS  Google Scholar 

  22. Henry, E. W. & Sidman, R. L. Science 241, 344–346 (1988).

    Article  ADS  CAS  Google Scholar 

  23. Levi, G., Crossin, K. & Edelman, G. M. J. Cell Biol. 105, 2359–2372 (1987).

    Article  CAS  Google Scholar 

  24. Chomczynski, P. & Sacchi, N. Analyt. Biochem. 162, 156–159 (1987).

    Article  CAS  Google Scholar 

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Topilko, P., Schneider-Maunoury, S., Levi, G. et al. Krox-20 controls myelination in the peripheral nervous system. Nature 371, 796–799 (1994). https://doi.org/10.1038/371796a0

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