Research reportInduction of antibodies against human prion proteins (PrP) by DNA-mediated immunization of mice
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A seven-residue deletion in PrP leads to generation of a spontaneous prion formed from C-terminal C1 fragment of PrP
2020, Journal of Biological ChemistryCitation Excerpt :Each mutant construct was verified by sequencing. The anti-PrP mAbs used were as follows: 4F2 directed to the octa-repeat domain (residues 62–94 according to sheep PrP numbering) (61, 62), 12B2 (residues 93–97) (63), 8G8 (residues 100–105) (61, 62), 6C2 (residues 114–121) (64) (Central Veterinary Institute, Wageningen University & Research, Wageningen, The Netherlands) Sha31 (residues 148–155) (62) (Bertin Pharma), and 8F9 (residues 224–234) (65). Sha31and 8G8 were biotinylated and further detected with horseradish peroxidase–conjugated streptavidin.
Generation and characterization of polyclonal antibodies against mouse T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory domain by DNA-based immunization
2014, Transplantation ProceedingsCitation Excerpt :The mTIGIT sequence was inserted into the multiple clone sites of pGM-T using a pGM-T clone kit (Tiangen, Beijing, China). The corresponding ligated products were digested with EcorI and XhoI, and then cloned into the commercially available mammalian expression vector pcDNA3.1 (+) [8–10]. The recombinant plasmids were transformed into Escherichia coli TOP10 cells (Tiangen, Beijing, China) to screen out the positive clone on LB agar with ampicillin resistance (100 μg/mL).
Rapid screening and confirmatory methods for biochemical diagnosis of human prion disease
2011, Journal of Virological MethodsCitation Excerpt :The monoclonal antibodies used in these assays were selected for their sensitive detection of the PK-treated and denatured PrP. The capture antibody 3B5 recognises the octarepeat region of PrP (Krasemann et al., 1996, 1999), while the tracer antibody 12F10 binds to the core part of the protein (Feraudet et al., 2005). PK digestion is carried out in a controlled concentration and a controlled medium (mixture of detergent and chaotropic agents) to preserve the N-terminal epitope.
Inducible expression of chimpanzee prion protein (PrP) in murine PrP knock-out cells
2010, Protein Expression and PurificationPolyclonal anti-idiotypic antibodies which mimic an epitope of the human prion protein
2009, Molecular ImmunologyDiscrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody
2007, Journal of Virological Methods