Suppression of MAP2 in cultured cerebeller macroneurons inhibits minor neurite formation

doi:10.1016/0896-6273(92)90025-9

Neuron

Volume 9, Issue 4, October 1992, Pages 607-618

https://doi.org/10.1016/0896-6273(92)90025-9 Get rights and content

Abstract

We show here that antisense MAP2 oligonucleotides inhibit neurite outgrowth in cultured cerebellar macroneurons. Unlike control neurons, which first extend a lamellipodial veil followed by a consolidation phase during which the cells extend minor neurites, MAP2-suppressed cells persist with lamellipodia and later become rounded. The induction of microtubules containing tyrosinated tubulin, which parallels neurite outgrowth in control neurons, was blocked under antisense conditions. The small but significant increase in acetylated microtubules was not affected. In contrast, the suppression of tau, which selectively blocks axonal elongation, completely prevented the increase of acetylated microtubules, but did not modify the induction of labile microtubules. These results suggest that MAP2 and tau have different functions: the initial establishment of neurites depends upon MAP2, whereas further neurite elongation depends upon tau and microtubule stabilization.

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Neuron

ArticleSuppression of MAP2 in cultured cerebeller macroneurons inhibits minor neurite formation

Abstract

Neuroscience

Cell

Dev. Brain Res.

Dev. Brain Res.

Dev. Brain Res.

Trends Neurosci.

J. Biol. Chem.

Neuron

J. Biol. Chem.

Cell

Brain Res. Rev.

Trophic interactions between astroglial cells and hippocampal neurons in culture

Science

Initial phase of dendritic growth: evidence for the involvement of high molecular weight microtubule-associated proteins (HMWP) before the appearance of tubulin

J. Cell Biol.

Light and electron microscopic studies of the distribution of microtubule-associated protein 2 in rat brain: a difference between dendritic and axonal cytoskeletons

J. Comp. Neurol.

Differences in the cevelopmental patterns of three microtubule-associated proteins in the rat cerebellum

J. Neurosci.

The distribution of tau in the mammalian central nervous system

J. Cell Biol.

Regulation of microtubule composition and stability during nerve growth factor promoted neurite outgrowth

J. Cell Biol.

PC12 cells express juvenile microtubule-associated proteins during nerve growth factor-induced reurite outgrowth

J. Cell Biol.

Inhibition of neurite polarity by tau antisense oligonucleotides in primary cerebellar neurones

Nature

Differential subcellular localization of tubulin and the microtubule associated protein MAP-2 in brain tissue as revealed by immunocytochemistry with hybridoma monoclonal antibodies

J. Neurosci.

The effect of tau antisense oligonucleotides on neurite formation of cultured cerebellar macroneurons

J. Neurosci.

A comprehensive set of sequence analysis programs for the VAX

Nucl. Acids Res.

Nucleotide and amino acid sequences of embryonic rat MAP2c

Nucl. Acids Res.

Article
Suppression of MAP2 in cultured cerebeller macroneurons inhibits minor neurite formation