Elsevier

Neuroscience

Volume 52, Issue 2, January 1993, Pages 281-294
Neuroscience

Localization of GABA-like immunoreactivity in the monkey amygdala

https://doi.org/10.1016/0306-4522(93)90156-AGet rights and content

Abstract

Neurons exhibiting GABA-like immunoreactivity were identified in the monkey amygdala using an avidin-biotin immunohistochemical technique. The pattern of GABA immunoreactivity was very similar in the basolateral and superficial amygdaloid nuclei. In these regions GABA-positive cells were nonpyramidal neurons that were often arranged in clusters or curvilinear rows. These GABA-positive nonpyramidal neurons constituted about 25% of the total neuronal population of the basolateral and superficial amygdaloid nuclei. Numerous GABA-positive puncta resembling axon terminals were observed both in the neuropil and encapsulating the perikarya of GABA-negative pyramidal cells.

The pattern of GABA-like immunoreactivity was different in the central and medial amygdaloid nuclei. These regions contained a very dense array of GABA-positive puncta. There were numerous GABA-positive neurons in the lateral subdivision of the central nucleus and fewer cells in the medial nucleus and medial subdivision of the central nucleus. Many immunoreactive puncta were observed contacting the perikarya and dendrites of GABA-positive cells in these regions. The intercalated nuclei consisted of numerous, small, GABA-positive neurons and a few, larger, GABA-negative cells. Both cell types were contacted by GABA-positive puncta.

This study indicates that neuronal subpopulations in each of the amygdaloid nuclei of the monkey are GABAergic. The pattern of immunoreactivity varies in different amygdaloid regions and is very similar to that described in the rat. Certain aspects of the functional organization of this rich GABAergic circuitry can be elucidated by correlating the findings of the present investigation with previous anatomical, physiological, and pharmacological studies of the amygdala.

References (65)

  • Le Gal La SalleG. et al.

    Neurochemical mapping of GABAergic systems in the amygdaloid complex and bed nucleus of the stria terminalis

    Brain Res.

    (1978)
  • LoscherW. et al.

    Further evidence for abnormal GABAergic circuits in amygdala-kindled rats

    Brain Res.

    (1987)
  • MarcelD. et al.

    Benzodiazepine binding sites: localization and characterization in the limbic system of the rat brain

    Brain Res Bull.

    (1986)
  • McDonaldA.J.

    Cytoarchitecture of the nucleus of the lateral olfactory tract: a Golgi study in the rat

    Brain Res. Bull.

    (1983)
  • McDonaldA.J.

    Neurons of the bed nucleus of the stria terminalis: a Golgi study in the rat

    Brain Res. Bull.

    (1983)
  • McDonaldA.J.

    Immunohistochemical identification of gamma-aminobutyric acid-containing neurons in the rat basolateral amygdala

    Neurosci. Lett.

    (1985)
  • McDonaldA.J.

    Coexistence of somatostatin with neuropeptide Y, but not with cholecystokinin or vasoactive intestinal peptide, in neurons of the rat amygdala

    Brain Res.

    (1989)
  • McDonaldA.J. et al.

    Co-localization of glutamate and tubulin in putative excitatory neurons of the hippocampus and amygdala: an immunohistochemical study using monoclonal antibodies

    Neuroscience

    (1989)
  • McDonaldA.J. et al.

    Coexistence of GABA and peptide immunoreactivity in non-pyramidal neurons of the basolateral amygdala

    Neurosci. Lett.

    (1989)
  • MillhouseO.E. et al.

    Neuronal configurations in lateral and basolateral amygdala

    Neuroscience

    (1983)
  • NagyJ. et al.

    Location of the site of the tranquillizing action of diazepam by intralimbic application

    Neuropharmacology

    (1973)
  • NagyJ. et al.

    Anti-anxiety action of diazepam after intra-amygdaloid application in the rat

    Neuropharmacology

    (1979)
  • NagyJ. et al.

    Further studies on the site of action of diazepam: anticonvulsant effect in the rabbit

    Neuropharmacology

    (1979)
  • NiehoffD.L. et al.

    Multiple benzodiazepine receptors: autoradiographic localization in normal human amygdala

    Brain Res.

    (1983)
  • OertelW.H. et al.

    Immunocytochemical studies of GABAergic neurons in rat basal ganglia and their relations to other neuronal systems

    Neurosci. Lett.

    (1984)
  • PetersenE.N. et al.

    Evidence that the anticonflict effect of midazolam in amygdala is mediated by the specific benzodiazepine receptors

    Neurosci. Lett.

    (1985)
  • PrelevicS. et al.

    A microelectrode study of amygdaloid afferents: temporal neocortical inputs

    Brain Res.

    (1976)
  • Scheel-KrugerJ. et al.

    Anticonflict effect of the benzodiazepines mediated by a GABAergic mechanism in the amygdala

    Eur. J. Pharmac.

    (1982)
  • TurskiW.A. et al.

    Injections of picrotoxin and bicuculline into the amygdaloid complex of the rat: and electroencephalographic, behavioral and morphological analysis

    Neuroscience

    (1985)
  • UemuraS. et al.

    Amygdaloid kindling with bicuculline methiodide in rats

    Expl Neurol.

    (1988)
  • UngerJ.W. et al.

    Neuropeptides and neuropathology in the amygdala in Alzheimer's disease: relationship between somatostatin, neuropeptide Y and subregional distribution of neuritic plaques

    Brain Res.

    (1988)
  • WieserH.G. et al.

    Selective amygdalohippocampectomy as a surgical treatment of mesiobasal limbic epilepsy

    Surg. Neurol.

    (1982)
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