Regular ArticleCell Types Required to Efficiently Innervate Human Muscle Cells in Vitro
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Cited by (25)
Neuromuscular junction formation between human stem cell-derived motoneurons and human skeletal muscle in a defined system
2011, BiomaterialsCitation Excerpt :In addition, human ESC-derived MNs have been investigated for their capability of innervating C2C12 cells in a serum-containing system [30–34], and MNs derived from human fetal spinal cord stem cells were demonstrated to be able to form functional NMJs with rat myotubes derived from embryonic skeletal muscles in a defined serum-free system [27]. Separately, cloned human skeletal muscle satellite cells have been used extensively for the study of in vitro NMJ formation or related diseases in combination with rat spinal explants or dissociated MNs in serum-containing systems [30–34]. In this study, we endeavored to develop an entirely human-based in vitro neuromuscular junction system, in which both MNs and SKMs were derived from stem cells, in a defined, serum-free system.
Node of Ranvier formation on motoneurons in vitro
2009, BiomaterialsCitation Excerpt :This also is an essential requirement for drug discovery applications. Furthermore, the reported importance of culturing motoneurons, sensory neurons and Schwann cells together with muscle to form a significant number of neuromuscular junctions in vitro makes this basic medium even more critical [24,25]. Schwann cell interaction with axons in the periphery is essential for efficient myelin sheath formation.
Synaptogenetic mechanisms controlling postsynaptic differentiation of the neuromuscular junction are nerve-dependent in human and nerve-independent in mouse C2C12 muscle cultures
2008, Chemico-Biological InteractionsCitation Excerpt :Stronger nerve-dependency of human muscle is not surprising. Our previous observations [17,19,20] as well as reports from other laboratories [16,24] agree that human myotubes never contract spontaneously, remain poorly differentiated and ultimately die if they are not innervated. On the other hand rodent muscle contract spontaneously and reach high level of differentiation without any presence of the nerve.
Expression and distribution of acetylcholinesterase among the cellular components of the neuromuscular junction formed in human myotube in vitro
2005, Chemico-Biological InteractionsCitation Excerpt :The temporal coincidence between maturation of the NMJ and lineage progression of neurons and glial cells was found to be practically the same as observed in vivo [5]. Important insight into the intercellular relationships in this coculture was provided by Guettier-Sigrist et al. [6]. They found that rat motor neurons alone were unable to innervate human myotubes and that three cell types (motoneurons, sensory neurons and Schwann cells) are required for a successful functional innervation.
Schwann cells and astrocytes induce synapse formation by spinal motor neurons in culture
2004, Molecular and Cellular NeuroscienceCitation Excerpt :These authors found that trophic factors that increase Xenopus survival of motor neurons inhibit synaptogenesis, but that this inhibition can be overcome by Schwann cell-conditioned media. The present findings, however, show that Schwann cells secrete a signal that strongly and specifically promotes synaptogenesis between SMNs, but is not sufficient to promote synaptogenesis between rat motor neurons and muscle in culture, perhaps because other cell types or signals are also required for rat NMJ synapse formation (Guettier-Sigrist et al., 2000). Two recent studies provide evidence that Schwann cells in vivo are important for the formation and maintenance of NMJ synapses.
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