Thermodynamic properties of calcium binding to C2AWT using ITC
| KD (μm) | ΔH (cal/mol) | ΔS (cal/mol/K) | ΔG (kcal/mol) |
|---|---|---|---|
| KD1 = 20.7 ± 3.9 | ΔH1 = 1005 ± 72.3 | ΔS1 = 24.9 ± 0.12 | ΔG1 = −6.17 |
| KD2 = 83.4 ± 19 | ΔH2 = 407.5 ± 166 | ΔS2 = 20.1 ± 0.35 | ΔG2 = −5.38 |
| KD3 = 766 ± 302 | ΔH3 = 2406 ± 597 | ΔS3 = 22.7 ± 1.3 | ΔG3 = −4.13 |
Data represent mean ± SD, n = 3. KD, Dissociation constant; Δ, change in; H, enthalpy; S, entrophy; G, Gibbs free energy.