Interactions of MOR with Gα subunits
| Basal | Fentanyl | Morphine | |
|---|---|---|---|
| MOR/Gαi1 | + | ++ | ++ |
| MOR/Gαi2 | + | ++ | +++ |
| MOR/Gαi3 | + | + | +++ |
| MOR/Gαq | + | +++ | + |
| MOR/Gαo | + | ++ | ++ |
Mice were treated with saline, morphine (20 mg/kg), or fentanyl (0.125 mg/kg) for 30 min. Striatal extracts were immunoprecipitated with an anti-MOR antibody, and the immunoprecipitate was immunoblotted for Gαi1, Gαi2, Gαi3, Gαq, or Gαo. Each experiment was performed at least three times.