Primers used for prestin mutagenesis
| Primer | Sequence |
|---|---|
| PrestinWH1Fwd | 5′TTCAGCTTCCCCAAGGCTTATGGTTCTGGATGTGGGCGGCTGTTCC |
| PrestinWH1Rev | 5′TAAGCCTTGGGGAAGCTGAAGCACGCCGGT |
| PrestinWH2Fwd | 5′CTGTTCCTCCGGTGTTCGGCTGGTACTCTTCATGGTATCCTGTTAT |
| PrestinWH2Rev | 5′GCCGAACACCGGAGGAACAGCCGCGAGCAT |
| A100WFwd | 5′TTCCCCAAGGCTTATGGTTCGCAATGCTCGC |
| A100WRev | 5′GAGCATTGCGAACCATAAGCCTTGGGGAAGC |
| A100VFwd | 5′TTCCCCAAGGCTTAGTGTTCGCAATGCTCGC |
| A100VRev | 5′GAGCATTGCGAACACTAAGCCTTGGGGAAGC |
| A100LFwd | 5′TTCCCCAAGGCTTACTGTTCGCAATGCTCGC |
| A100LRev | 5′GAGCATTGCGAACAGTAAGCCTTGGGGAAGC |
| A100GFwd | 5′TCCCCAAGGCTTAGGATTCGCAATGCTCGC |
| A100GRev | 5′GAGCATTGCGAATCCTAAGCCTTGGGGAAGC |
| A100SFwd | 5′TTCCCCAAGGCTTATCCTTCGCAATGCTCGC |
| A100SRev | 5′GCGAGCATTGCGAAGGATAAGCCTTGGGG |
| A102WFwd | 5′CTTAGCCTTCTGGATGCTCGCGGCTGTTCC |
| A102WRev | 5′CAGCCGCGAGCATCCAGAAGGCTAAGCC |
| A102GFwd | 5′CTTAGCCTTCGGAATGCTCGCGGCTGTTC |
| A102Grev | 5′CAGCCGCGAGCATTCCGAAGGCTAAGCC |
| A102VFwd | 5′CTTAGCCTTCGTGATGCTCGCGGCTGTTCC |
| A102GRev | 5′CAGCCGCGAGCATCACGAAGGCTAAGCC |
| A102LFwd | 5′CTTAGCCTTCCTGATGCTCGCGGCTGTTCC |
| A102LRev | 5′CAGCCGCGAGCATCAGGAAGGCTAAGCC |
| A102SFwd | 5′GCTTAGCCTTCTCCATGCTCGCGGCTGTTC |
| A102SRev | 5′GAACAGCCGCGAGCATGGAGAAGGCTAAGC |
| L104WFwd | 5′CCTTCGCAATGTGGGCGGCTGTTCCTC |
| L104Wrev | 5′GAACAGCCGCCCACATTGCGAAGGCTAAGC |
| L104VFwd | 5′CCTTCGCAATGGTCGCGGCTGTTCCTC |
| L104VRev | 5′GAGGAACAGCCGCGACCATTGCGAAGG |
| L104IFwd | 5′CCTTCGCAATGATCGCGGCTGTTCCTC |
| L104Rev | 5′GAGGAACAGCCGCGATCATTGCGAAGG |
| L113WFwd | 5′CTCCGGTGTTCGGCTGGTACTCTTCATTTTATCCTG |
| L113Wrev | 5′GGATAAAATGAAGAGTACCAGCCGAACACCGGAGG |
| F117WFwd | 5′GTGTTCGGCCTGTACTCTTCATGGTATCCTGTTATCATGTACTG |
| F117WRev | 5′GTACATGATAACAGGATACCATGAAGAGTACAGGCCGAACAC |
| A305VFwd | 5′GGAACTGGCATTTCCGTGGGGTTTAACTTGC |
| A305VRev | 5′GCAAGTTAAACCCCACGGAAATGCCAGTTC |
Fwd, Forward; Rev, reverse.