Gene | K252a (tyr kinase) | U0126 (MEK) | PD98059 (MEK) | U73122 (PLC-γ) | LY294002 (PI3 kinase) | KN93 (CaMKII) | H-89 (PKA) | CC (PKC) |
---|---|---|---|---|---|---|---|---|
20 min | ||||||||
c-fos | Blocked | Blocked | Blocked | Blocked | UC | Blocked | UC | UC |
EGR1 | Blocked | Blocked | Blocked | UC | UC | Blocked | UC | UC |
Arc | Blocked | Blocked | Blocked | Blocked | UC | Blocked | UC | UC |
3 hr | ||||||||
VGF | Blocked | Blocked | Blocked | Blocked | UC | Blocked | UC | UC |
The regulation of BDNF-induced genes in the presence of inhibitors was determined by real time RT-PCR. Neurons were preincubated with inhibitors for 30 min before BDNF treatment (20 min for IEGs and 3 hr for VGF). All samples were first normalized to GAPDH and then represented as a ratio of vehicle controls. One group of cells in each experiment was treated with inhibitor alone and showed no significant change in expression relative to vehicle control. “Blocked” indicates significant reduction of transcription in the presence of inhibitor plus BDNF relative to BDNF alone (p <0.05; ANOVA; n=3). UC (unchanged) indicates no significant effector of inhibitor on transcription. K242a, U0126, PD98059, U73122, and KN93 blocked BDNF-induced transcription of the IEGs and VGF, whereas LY294002, H-89, and CC did not affect gene expression.