Clone number | Gene/Protein name | Abbreviation | Accession number | Fold change | NGF |
---|---|---|---|---|---|
20 min BDNF | |||||
A10g | c-fos proto-oncogene | c-fos | X06769 | 2.2 ± 0.4 | UC |
E06d | Early growth response protein 1 | EGR1 | M18416 | 4.3 ± 0.8 | UC |
F13I | Activity-regulated cytoskeletal-associated | Arc | U19866 | 13.7 ± 5.0 | UC |
3 hr BDNF | |||||
A05d | fos-related antigen 2 | Fra2 | U18913 | 2.3 ± 0.4 | down |
A07a | G1/S-specific cyclin D1 | cyclin D1 | D14014 | 3.3 ± 0.5 | UC |
A10g | c-fos proto-oncogene | c-fos | X067679 | 2.3 ± 0.4 | UC |
B05d | Voltage-gated potassium channel protein | Kv + 1.1 | M26161 | 1.4 ± 0.1 | UC |
B06j | Sodium channel, beta 1 subunit | Na + ch | M91808 | 1.8 ± 0.2 | UC |
B13n | Secretogranin II precursor | Sgll | M93669 | 5.1 ± 1.2 | UC |
D04i | Somatostatin receptor 4 | SSTR4 | U04738 | 1.7 ± 0.1 | down |
D11k | Transmembrane receptor UNC5 homolog | UNC5H2 | U87306 | 2.2 ± 0.6* | UC |
D13I | Neuropeptide Y precursor | NPY | M20373 | 1.9 ± 0.3* | down |
D14f | VGF protein precursor | VGF | M60525 | 6.5 ± 1.6 | UC |
E06d | Early growth response protein 1 | EGR1 | M18416 | 6.6 ± 0.9 | UC |
E12f | Protein-tyrosine phosphatase 1B | PTP1B | M33962 | 1.4 ± 0.1 | UC |
F13I | Activity-regulated cytoskeletal-associated | Arc | U19866 | 34.8 ± 4.3 | UC |
Two independent Atlas arrays were performed using RNA from hippocampal cells treated for either 20 min or 3 hr with BDNF (50 ng/ml) and control sister cultures. The criteria for selection from the array was a > 1.7-fold change. Real time RT-PCR validated the induction of 16 genes (average ± SE; n = 6; *n = 5; p < 0.05; t test). NGF was used as a neurotrophin control that does not induce plasticity. All gene changes appear to be specific to BDNF. UC, Unchanged; down, decreased.