TY - JOUR T1 - Distinct Intracellular Calcium Transients in Neurites and Somata Integrate Neuronal Signals JF - The Journal of Neuroscience JO - J. Neurosci. SP - 5344 LP - 5353 DO - 10.1523/JNEUROSCI.22-13-05344.2002 VL - 22 IS - 13 AU - Friedrich W. Johenning AU - Michal Zochowski AU - Stuart J. Conway AU - Andrew B. Holmes AU - Peter Koulen AU - Barbara E. Ehrlich Y1 - 2002/07/01 UR - http://www.jneurosci.org/content/22/13/5344.abstract N2 - Intracellular calcium signals have distinct temporal and spatial patterns in neurons in which signal initiation and repetitive spiking occurs predominantly in the neurite. We investigated the functional implications of the coexpression of different isoforms of ryanodine receptors (RyR) and inositol 1,4,5-trisphosphate receptors (InsP3Rs) using immunocytochemistry, Western blotting, and calcium imaging in neuronally differentiated PC12 cells. InsP3R type III, an isoform that has been shown to be upregulated in neuronal apoptosis, is exclusively expressed in the soma, serving as a gatekeeper for high-magnitude calcium surges. InsP3R type I is expressed throughout the cell and can be related to signal initiation and repetitive spiking in the neurite. RyR types 2 and 3 are distributed throughout the cell. In the soma, they serve as amplifying molecular switches, facilitating recruitment of the InsP3R type III-dependent pool. In the neurite, they decrease the probability of repetitive spiking. Use of a cell-permeant analog of InsP3 suggested that regional specificity in InsP3 production and surface-to-volume effects play minor roles in determining temporal and spatial calcium signaling patterns in neurons. Our findings suggest that additional modulatory processes acting on the intracellular channels are necessary to generate spatially specific calcium signaling. ER -