TY - JOUR T1 - Reduction of CuZn-Superoxide Dismutase Activity Exacerbates Neuronal Cell Injury and Edema Formation after Transient Focal Cerebral Ischemia JF - The Journal of Neuroscience JO - J. Neurosci. SP - 4180 LP - 4189 DO - 10.1523/JNEUROSCI.17-11-04180.1997 VL - 17 IS - 11 AU - Takeo Kondo AU - Andrew G. Reaume AU - Ting-Ting Huang AU - Elaine Carlson AU - Kensuke Murakami AU - Sylvia F. Chen AU - Eric K. Hoffman AU - Richard W. Scott AU - Charles J. Epstein AU - Pak H. Chan Y1 - 1997/06/01 UR - http://www.jneurosci.org/content/17/11/4180.abstract N2 - Apoptotic neuronal cell death has recently been associated with the development of infarction after cerebral ischemia. In a variety of studies, CuZn-superoxide dismutase (CuZn-SOD) has been shown to protect the brain from ischemic injury. A possible role for CuZn-SOD-related modulation of neuronal viability is suggested by the finding that CuZn-SOD inhibits apoptotic neuronal cell death in response to some forms of cellular damage. We evaluated this possibility in the model of transient focal cerebral ischemia in mice bearing a disruption of the CuZn-SOD gene (Sod1). Homozygous mutant (Sod1−/−) mice had no detectable CuZn-SOD activity, and heterozygous mutants (Sod1 +/−) showed a 50% decrease compared with wild-type mice. Sod1 −/− mice showed a high level of blood–brain barrier disruption soon after 1 hr of middle cerebral artery occlusion and 100% mortality at 24 hr after ischemia.Sod1 +/− mice showed 30% mortality at 24 hr after ischemia, and neurological deficits were exacerbated compared with wild-type controls. The Sod1 +/− animals also had increased infarct volume and brain swelling, accompanied by increased apoptotic neuronal cell death as indicated by the in situnick-end labeling technique to detect DNA fragmentation and morphological criteria. These results suggest that oxygen-free radicals, especially superoxide anions, are an important factor for the development of infarction by brain edema formation and apoptotic neuronal cell death after focal cerebral ischemia and reperfusion. ER -