Figure 2. Calcineurin and dynamin blockers inhibit endocytosis overshoot. A–D, Sampled ICa and Cm induced by 3 depol50 ms × 10 applied every 50 s in the presence of CaN457–482 (CaNp, 150 μm in pipette, A), scrambled CaN457–482 (SCaNp, 150 μm, B), CsA (20 μm in pipette, C), or dynasore (100 μm in pipette, D). Cm change induced by the third and the first depol50 ms × 10 was superimposed (middle). Subtraction of the third Cm response from the first one yields a trace reflecting endocytosis overshoot only (right). E, Sampled ICa and Cm induced by a depol50 ms × 10 in the presence of GDPβS (0.3 mm in pipette). F, The overshoot amplitude induced by the first depol50 ms × 10, calculated as the difference between the first and the third Cm response (A–D), plotted in the presence of CaNp (n = 7), SCaNp (n = 7), CsA (n = 9) or dynasore (Dyn, n = 15).