Figure 6.
Lamina-restricted arbors of RGC axons within the retinorecipient zone of the superior colliculus.
a–j
, Vibratome sections were immunostained with anti-GFP (green) and counterstained with Neurotrace or TO-PRO (blue).
a
,
c
,
e
,
g
,
i
, Coronal sections from mice that had been monocularly enucleated to ablate retina axons from the side marked “ipsi.”
b
,
d
,
f
,
h
,
j
, Sagittal sections from other mice that had not been enucleated. Brackets indicate the span of axonal arbors of RGC subtypes. Transgenic lines are indicated at the left; mice were 40 d of age or older.
k
, The entire retinorecipient zone is marked by injection of cholera toxin b subunit (CTB, red) into one eye, and corresponds to that marked in Chx10-Cre × TSY mice in
a
. Arrows in
a
and
k
show sparse ipsilateral projections, which terminate in a deep region.
l
, Schematic showing distinct axonal restriction of each type of RGCs and ipsi projection. Scale bar: (in
k
)
a–k
, 300 μm.