Figure 2.
T286D-dependent depression is reversed by an NMDAR antagonist (APV), calcineurin inhibitor (FK-506), or additional mutation at T305A/T306A. Gray, Control condition; orange, T286D in nontreated culture medium; red, T286D in culture medium containing APV; brown, T286D in culture medium containing FK-506; green, triple mutant (T286D/T305A/T306A).
A
, The representative EPSC traces. Calibration: 50 ms, 50 pA.
B
, T286D-dependent depression is reversed by an NMDAR antagonist, calcineurin inhibitor, or additional mutation at T305A/T306A. T286D, Nontransfected = 117.1 ± 16.16 pA, transfected = 39.8 ± 8.59 pA, n = 14, **p < 0.01; T286D (APV), transfected = 66.1 ± 6.57 pA, nontransfected = 28.7 ± 6.41 pA, n = 8, **p < 0.01; T286D (FK506), transfected = 100.7 ± 10.44 pA, nontransfected = 37.1 ± 11.57 pA, n = 9, *p < 0.05; T286D/T305A/T306A, transfected = 80.1 ± 9.39 pA, nontransfected = 42.4 ± 7.51 pA, n = 18, *p < 0.05; T286D/T305D/T306D, transfected = 31.3 ± 4.95 pA, nontransfected = 74.5 ± 10.99 pA, n = 22, **p < 0.01.