Figure 1.
Amino acid sequences of four Dscam endodomain variants and silencing of various UAS-Dscam transgenes by specific UAS-RNAis. A, Amino acid sequences of four Dscam endodomain variants, full length (+19 +23), missing exon 19 only (−19 +23), lacking exon 23 only (+19 −23), and omitting both exon 19 and exon 23 (−19 −23), are aligned within the cytoplasmic domain from exon 18 to exon 24. Common sequence between exon 20 and exon 22 is not shown. Four extra amino acids (TVIS) are in the Dscam variants which skip exon 19. Amino acids shown in italic result from a reading frame shift in exon 24 upon skipping of exon 23. Target sequences for different UAS-RNAis (18i, 18/20i, 19i, 22/24i and 23i) to eliminate different Dscam variants are indicated. B–I, Composite confocal images of wandering larval MBs show suppression of the GAL4–201Y-dependent induction of UAS-Dscam+19 +23::GFP (B–E) and UAS-Dscam−19 −23::GFP (F–I) by UAS-18/20RNAi (B, F), UAS-19RNAi (C, G), UAS-22/24RNAi (D, H) and UAS-23RNAi (E, I), respectively. Scale bar (in this and all other figures), 50 μm.