Mouse monoclonal anti-neuron-specific nuclear protein (NeuN) | 1:100 | 36 h at 4°C | Chemicon | Postmitotic neurons |
Polyclonal rabbit anti-NG2 | 1:200 | ON at 4°C | Chemicon | Glial precursors |
Polyclonal rat BrdU | 1:100 | 36 h at 4°C | Oxford Biotechnology | Mitotic cells |
Monoclonal mouse anti-RECA-1 | 1:100 | 36 h at 4°C | Serotec | Endothelial cells |
Monoclonal mouse anti-PECAM-1/CD31 | 1:100 | ON at 4°C | Dr. Peter J. Newman | Endothelial cells |
Monoclonal mouse anti-EBA | 1:2000a; 1:1000b | ON at 4°C | Sternberger Monoclonals | Vessels with BBB properties |
Monoclonal mouse anti-nestin | 1:25a; 1:5000b | 36 h at 4°C | BD Pharmingen | Angiogenic vessels |
Polyclonal sheep anti-albumin | 1:60,000 | 36 h at 4°C | Biogenesis | Blood-borne albumin |
Monoclonal mouse anti-Ox42 (CD11b) | 1:100 | ON at 4°C | Serotec | Microglia |
Monoclonal mouse anti-GFAP | 1:100 | 36 h at 4°C | DakoCytomation | Astrocytes |
Polyclonal rabbit anti-TH | 1:100 | ON at 4°C | Pel-Freeze | DA neurons |