Fig. 2. Representative immunoblot of the effects of METH on DAT protein concentration. Lanes 1, 3, 5, From saline-injected +/+, +/−, and −/− c-fos mutant mice, respectively.Lanes 2, 4, 6, From METH-injected +/+, +/−, and −/− c-fos knock-out mice, respectively. There were significant changes in DAT concentration consisting of 44.6 ± 4.7, 57.9 ± 6.8, and 78.8 ± 5.3% decreases in +/+, −/−, and −/− c-fos knock-out mice, respectively. α-Tubulin is also shown and reveals similar loading for all groups (6 mice per group). Nt, Antibody against N terminus of the protein.